A hallmark characteristic of breast cancer cells is rapid proliferation and aberrant invasion, fueled by hijacking adipose endocrine and energy regulation functions. Isolating the bi-directional crosstalk between adipocytes and cancer cells from other stromal components necessitates engineering physiologically-relevant in vitro tissue models. A spatially-defined, 3-D co-culture system of differentiated human adipose cells and breast cancer cells was generated by Laser Direct-Write. Breast cancer cells, encapsulated in alginate-collagen microbeads, were deposited in a bead-by-bead manner into a matrix of differentiated adipocytes.
- Theresa Phamduy
Matthew E. Burrow, Ph.D. (Burrow Lab)
Molecular and Cellular Oncology Laboratory, Tulane Univeristy
Bruce A. Bunnell, Ph.D. (Faculty Page))
Director, Tulane Center for Stem Cell Research and Regenerative Medicine
Professor, Department of Pharmacology, Tulane University School of Medicine
Chairman, Division of Gene Therapy, Tulane National Primate Research Center