Effect of Pattern Spacing and Configuration on Breast Cancer Migration in Co-Culture with Fibroblasts
Patterned co-culture of FBs and BCCs. Groups of FBs and GFP-transfected BCCs were transferred into a 3x4 matrix pattern by laser direct-write. The center-to-center spacing in the pattern was 800 µm. The configuration depicted displays alternating columns of FBs and BCCs. At day 0, each individual spot contained only one cell type. Migration and infiltration of cancer cells were tracked up to seven days, post-transfer. 10X magnification, scale bar = 200 µm.