Laser Direct-Write

Description: Customized excimer UV laser system (ArF, 193 nm, near-Gaussian energy distribution, pulse width = ~8 ns, average power = 5W @ 500 Hz) for micron-scale materials processing. System is integrated with inline photometer for real-time energy readings and motorized iris for beam sizing. Independently-controlled motorized ribbon and substrate stages allow for computer-aided design/manufacturing feasibility. System also includes in situ camera for real-time video feed at laser focus and incubator for temperature and humidity control in working space.

Uses: (1) Patterning of cells, within resolution of ±5 um, (2) printing of single microbeads, (3) fabrication of hydrogel microbeads, (4) micromachining on metal or hydrogel materials

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Carl Zeiss Cell Observer with Apotome module

Description: Multi-modality epi-configuration microscope with temperature and CO2 control modules for live-cell timelapse imaging. Transmission light modes: bright field, phase contrast, differential interference contrast. Fluorescence filters: (Blue) Em = 390, Ex = 460; (Green) Em = 470, Ex = 525; (Red) Em = 565, Ex = 620 nm. Objective lens: 10x, 20x, 20xLD, 40x, 63x, 100x. Apotome module for confocal-like imaging.

Uses: (1) Multi-channel imaging in both transmission and wide-field fluorescent light, (2) Apotome imaging for optical slices, (3) mosaic imaging over large areas, (4) z-stack imaging of tissue samples, (5) live-cell timelapse imaging

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Ink Jet Printer

Description: The DMP-2831 inkjet printer can be used to inkjet print custom inks for materials research. Earlier materials research used repurposed consumer printers for this purpose. This inkjet printer gives much finer control over every aspect of the printing process allowing one to control the drop spacing, drop size, and the size and the shape of the signal sent to the piezoelectric controller. Disposable cartridges are used, which have a capacity of 1.5 ml. Since it is an inkjet printer, any inkjet printable pattern is possible, for example a circuit.

Uses: fabricating energy storage and energy conversion devices, such as capacitors or proton conducting membranes

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Spin Coater

Description: Spin coater platform capable of generating 1 um - 1 mm thick films of viscoelastic liquids by spinning up to 10,000 RPM. Max substrate sizes: ø150mm wafers and 4" × 4" (102mm × 102mm) substrates, with adaptor for smaller substrates.

Uses: coating surfaces with gelatin for LDW

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Image Analysis Workstation

Description: Workstation terminal (Windows 7, 64-bit, 8 G RAM, 3.7 GHz CPU) with dual 21" screen monitors. Programs installed: ImageJ, IPLab, AxioVision (offline version), Matlab, ECIS.

Uses: Semi-automated and automated image analysis

Microbead Fabrication Station

Description: Integrated high voltage source (up to 30 kV DC) and syringe pump (up to 519 mL/hr, dispensing volumes = 10 µL - 60 mL) for the ejection of liquids across an electric field.

Uses: (1) fabrication of hydrogel microbeads, (2) electro-spinning

Electrical Cell Impendence Sensing (ECIS)

Description: Electrical measurement instrument for automated, non-invasive monitoring of cell behavior in real-time without the use of labels. Instrument consists of a Zθ system controller, 16-channel array holder installed in a dedicated incubator, computer and integrated software running on Windows XP. Measures the complex impedance spectrum (Z, R, C, frequency range = 100 Hz - 64 kHz) of adherent cells growing on gold electrodes.

Uses: (1) real-time monitoring of cell behavior, (2) wound healing assays, (3) invasion assays

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